ABSTRACT
OBJECTIVE: To investigate the effects of oligosaccharide esters of Polygala tenuifolia on Aβ25-35 induced injury in SH-SY5Y cells and the relative mechanisms. METHODS: SH-SY5Y cells were cultured with Aβ25-35(25 μmol•L-1) for 24 h in the presence and absence of oligosaccharide esters component of Polygala tenuifolia. The cell viability was measured by MTT method; cell apoptosis was detected by flow cytometry; the LDH release, the SOD activity and MDA level were measured by biochemistry method; the Bcl-2 and Bax expression were determined by Western blotting. RESULTS: Oligosaccharide esters of Polygala tenuifolia could enhance the cell viability, decrease the apoptotic rate, the release of LDH and MDA level, and increase SOD activity. It also enhanced the expression of anti-apoptotic protein Bcl-2 and decreased the expression of apoptotic protein Bax. CONCLUSION: Oligosaccharide esters of Polygala tenuifolia have neuroprotective effects against the Aβ25-35 induced injury in SH-SY5Y cells, which is related to its anti-oxidation and anti-apoptosis.
ABSTRACT
Oligosaccharide esters have attracted more and more attention along with the study of the chemical constituents of Polygala tenuifolia. Modern pharmacological studies have shown that these compounds have many pharmacological activities, such as neuroprotection, antidepressant effect and improvement of learning ability and memory. This review summarized the research progress of pharmacological effects of oligosaccharide esters from Polygala tenuifolia to provide clues for further study of the neuroprotective effects of Polygala tenuifolia.